UC Santa Barbara’s retinal research reveals how neighboring cells regulate neuronal size, connectivity.
Thanks to a new study of the retina, scientists at UC Santa Barbara have developed a greater understanding of how the nervous system becomes wired during early development.
The findings reflect the expansion of developmental neurobiology and vision research at UC Santa Barbara. The work is described in a recent publication of the Journal of Neuroscience.
The research team examined the connectivity of nerve cells, called neurons, in mice. Neurons communicate with one another via synapses where the dendrites and axon terminals of different cells form contacts. This is where nerve signals are transmitted from one neuron to another.
Scientists have understood for some time how neuronal activation at developing synapses contributes to the patterns of connectivity observed in maturity, explained Ben Reese, senior author and professor in UC Santa Barbara’s Neuroscience Research Institute and the Department of Psychological & Brain Sciences.
Incoming activity plays a critical role in sculpting neuronal form and the elaboration of synaptic connections. The new research shows, by contrast, how relationships between neighboring cells of the same type independently regulate neuronal size and connectivity.
The researchers circumvented the difficulty of visualizing the three-dimensional relationships between neurons within the brain by working within the retina. The retina is an outgrowth of the brain during embryonic development, and is a precisely layered structure in which the cells, their dendrites and their axons are restricted to discrete strata. “This makes the visualization and analysis of neuronal morphology and connectivity far simpler,” said Reese.
The scientists used two genetically modified mouse models to modulate the density of one particular type of retinal neuron, a class of cone bipolar cell. Cone bipolar cells relay information from the population of cone photoreceptors to the retinal ganglion cells. The latter are neurons that in turn project information to locations within the brain where further visual processing of the retinal image takes place.
The lead author on the study, Sammy Lee, was a postdoctoral researcher working in Reese’s lab and supported by a C.J. Martin National Health & Medical Research Council fellowship from Australia during the course of the study. Lee labeled individual cone bipolar cells with a fluorescent dye through a new microinjection procedure developed by Patrick Keeley, a graduate student in the Reese lab.
“What Dr. Lee has shown is that cone bipolar cells modulate the size of their dendritic fields (branched extensions of the neuron) in association with the local density of like-type neurons,” said Reese. “One line of mice has conspicuously fewer cone bipolar cells, each now with a larger dendritic territory, while the other line shows heightened densities and correspondingly smaller dendritic fields.”